Simplified adenine base editors improve adenine base editing efficiency in rice
نویسندگان
چکیده
منابع مشابه
Modelling proton tunnelling in the adenine-thymine base pair.
The energies of the canonical (standard, amino-keto) and tautomeric (non-standard, imino-enol) charge-neutral forms of the adenine-thymine base pair (A-T and A*-T*, respectively) are calculated using density functional theory. The reaction pathway is then computed using a transition state search to provide the asymmetric double-well potential minima along with the barrier height and shape, whic...
متن کاملDNA base stacking involving adenine and 2-aminopurine
The potential energy surfaces of stacked structures consisting of adenine (A) and 2-aminopurine (2AP) have been investigated in the gas phase. Both faceto-back (the double-ring system of one base exactly on top of that of the other one) and face-to-face (one base flipped by 180 ) A/A, 2AP/2AP and A/2AP stacks were considered. Minima and transition states were optimised at the counterpoise-corre...
متن کاملQuantum-chemical ab initio study on the adenine-difluorotoluene complex--a mimic for the adenine-thymine base pair.
Recent experiments have shown that difluorotoluene (F), a nonpolar isostere for thymine (T), codes efficiently and specifically for adenine (A) in DNA replication. F has almost the same shape as thymine but it is unable to form conventional hydrogen bonds with adenine. Therefore, it has been claimed that not hydrogen bonding but shape complementary may be important for the selection of the corr...
متن کاملCharacterization of photophysical and base-mimicking properties of a novel fluorescent adenine analogue in DNA
To increase the diversity of fluorescent base analogues with improved properties, we here present the straightforward click-chemistry-based synthesis of a novel fluorescent adenine-analogue triazole adenine (A(T)) and its photophysical characterization inside DNA. A(T) shows promising properties compared to the widely used adenine analogue 2-aminopurine. Quantum yields reach >20% and >5% in sin...
متن کاملInfrared Signatures of Proton Transfer in Guanine⋅Cytosine and Adenine⋅Thymine Base Pairs: DFT Study
The structures of all stable tautomers of guanine⋅cytosine and adenine⋅thymine Watson–Crick base pairs produced by single and double proton transfers from the most stable structures are optimized using density functional theory (DFT) at the B3LYP/6-31G(d,p) level. The zwitterionic tautomer of guanine⋅cyosine is stable in a high-dielectric medium (water), but not in the gas phase. Normal mode ca...
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ژورنال
عنوان ژورنال: Plant Biotechnology Journal
سال: 2019
ISSN: 1467-7644,1467-7652
DOI: 10.1111/pbi.13244